Journal: Nature Communications

Article Title: TWEAK mediates inflammation in experimental atopic dermatitis and psoriasis

doi: 10.1038/ncomms15395

Figure Lengend Snippet: ( a ) Basal surface expression of Fn14 (line) on NHEK cells relative to Isotype staining (grey). ( b , c ) NHEK cells were stimulated for 48 h with rTWEAK, rIL-13, rIL-17 or their combination. mRNA expression of indicated chemokines was assessed relative to GAPDH. Means of triplicate cultures with standard deviations. Values were compared using Student's t -test. One out of three independent experiments.

Article Snippet: Cells were cultured in 6 and 12 well format and stimulated with rTWEAK (400 ng ml −1 murine rTWEAK for PAM212, 25-100 ng ml −1 human rTWEAK for human cells), 100 ng ml −1 species-specific rIL-13, 100 ng ml −1 species-specific rIL-17A, or combinations thereof (all from Peprotech, Rocky Hill, NJ).

Techniques: Expressing, Staining

Journal: Nature Communications

Article Title: TWEAK mediates inflammation in experimental atopic dermatitis and psoriasis

doi: 10.1038/ncomms15395

Figure Lengend Snippet: ( a , c ) Naive WT mice were injected with rTWEAK or IgG administered once s.c. After 4 days expression of mRNA for TSLP and IL-19 was measured in skin biopsies. Data relative to GAPDH. Combined results from two experiments. ( b , d ) WT and TWEAK-deficient animals were treated with HDM for 4 days ( b ) or IMQ for 8 days ( d ) and mRNA for TSLP and IL-19, respectively, assessed in skin, relative to GAPDH expression. One out of three experiments shown. ( e , f ) NHEK cells (left) or NHDF cells (right) were stimulated for 48 h or the indicated times with rTWEAK. mRNA expression of TSLP ( e ) and IL-19 ( f ) was assessed relative to GAPDH. Each data point in a – d represents one mouse. Bars represent mean values. The Mann–Whitney U -test was performed to compare the groups. Bars in ( e , f ) indicate mean values of triplicates and s.d. Values were compared using Student's t- test. One out of three independent experiments. Asterisks indicate P <0.05.

Article Snippet: Cells were cultured in 6 and 12 well format and stimulated with rTWEAK (400 ng ml −1 murine rTWEAK for PAM212, 25-100 ng ml −1 human rTWEAK for human cells), 100 ng ml −1 species-specific rIL-13, 100 ng ml −1 species-specific rIL-17A, or combinations thereof (all from Peprotech, Rocky Hill, NJ).

Techniques: Injection, Expressing, MANN-WHITNEY